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Updated: Jul 9, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
Recent advances in genome engineering by CRISPR technology
Youngsik Lee1, Yeounsun Oh1, Seung Hwan Lee1
1Department of Life Science, Chung-Ang University, Seoul 06974, Korea.
Abstract:
Due to the development of CRISPR technology, the era of effective editing of target genes has arrived. However, the offtarget problem that occurs when recognizing target DNA due to the inherent nature of CRISPR components remains the biggest task to be overcome in the future. In this review, the principle of inducing such unintended off-target editing is analyzed from the structural aspect of CRISPR, and the methodology that has been developed to reduce off-target editing until now is summarized. [BMB Reports 2024; 57(1): 12-18].
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