Injectable and Microporous Microgel-Fiber Granular Hydrogel Loaded with Bioglass and siRNA for Promoting Diabetic
Ying Li1, Wei Song2, Lingzhi Kong2
1Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, 600 Yishan Road, Shanghai, 200233, China.
Small (Weinheim an Der Bergstrasse, Germany)
|December 6, 2023
Summary
This study introduces a novel microfiber-gel (MFgel) hydrogel for diabetic wound healing. MFgel enhances cell infiltration and promotes faster healing by regulating inflammation and MMP-9 expression.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Wound Healing
Background:
- Injectable hydrogels are vital for diabetic wound healing but face limitations with nano-porous structures hindering cell migration.
- Effective management of inflammation and matrix metalloproteinase-9 (MMP-9) is critical for successful diabetic wound repair.
Purpose of the Study:
- To develop an injectable, microporous granular microgel-fiber hydrogel (MFgel) for enhanced diabetic wound healing.
- To evaluate MFgel's potential in promoting cell infiltration, regulating inflammation, and accelerating tissue repair.
Main Methods:
- A novel method was used to produce microfiber-gel granules encapsulating bioceramics powders.
- MFgel was fabricated from hyaluronic acid (HA) and sodium alginate (SA) loaded with small interfering RNA (siRNA) and bioglass (BG) particles.
- In vitro and in vivo studies were conducted in mice and diabetic rats to assess MFgel's efficacy.
Main Results:
- MFgel exhibited a micron-sized porous structure, facilitating superior cell adhesion and penetration compared to traditional hydrogels (Tgel).
- MFgel demonstrated enhanced mechanical stability and promoted cellular/tissue infiltration and proliferation in vivo.
- Application of MFgel to diabetic rat skin defects accelerated healing by regulating inflammation, suppressing MMP-9, and enhancing angiogenesis and collagen deposition.
Conclusions:
- The developed MFgel hydrogel offers a promising solution for diabetic wound healing due to its unique microporous structure and therapeutic properties.
- MFgel effectively overcomes the limitations of traditional hydrogels, showing significant potential for clinical applications in managing complex wounds.
