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Updated: Jun 18, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
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A CRISPRi-based genetic resource to study essential Staphylococcus aureus genes

Patricia Reed1, Moritz Sorg1, Dominik Alwardt1

  • 1Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.

Mbio
|December 6, 2023
PubMed
Summary

Researchers optimized a clustered regularly interspaced short palindromic repeat interference (CRISPRi) system for Staphylococcus aureus. This system enabled the creation of a mutant library to study essential gene functions and combat antibiotic resistance.

Keywords:
CRISPRiStaphylococcus aureusessential genes

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