Related Experiment Video
Updated: Jul 9, 2025

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
Published on: March 31, 2021
Management of post-mortem examination in SARS-CoV-19 infections
Mattia Riefolo1,2, Francesca Ambrosi2,3, Alessandra De Palma4
1Pathology Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy.
Abstract:
A brief overview on the management of autopsies during the SARS-CoV-19 epidemic is proposed. In particular, the point is made of the Italian laws on the subject, the characteristics required for the autopsy room and the sampling suggested for the histological examination.
Insights
Guidelines for managing autopsies during the SARS-CoV-19 pandemic are presented, covering Italian legal requirements, autopsy room specifications, and histological sampling techniques for accurate examination.
Area of Science:
- Forensic Pathology
- Infectious Disease Management
Background:
- The SARS-CoV-19 pandemic necessitated specific protocols for post-mortem examinations.
- Standard autopsy procedures required adaptation to mitigate transmission risks.
Purpose of the Study:
- To provide an overview of autopsy management during the SARS-CoV-19 epidemic.
- To address legal, logistical, and technical aspects of conducting autopsies in the context of COVID-19.
Main Methods:
- Review of Italian legislation pertaining to autopsies during public health emergencies.
- Description of essential characteristics for autopsy suites handling infectious cases.
- Recommendations for tissue sampling for histological analysis.
Main Results:
- Outlined legal framework for SARS-CoV-19 related autopsies in Italy.
- Detailed requirements for autopsy room safety and infrastructure.
- Specified histological sampling strategies for effective disease characterization.
Conclusions:
- Adherence to specific legal and safety guidelines is crucial for SARS-CoV-19 autopsies.
- Appropriate histological sampling ensures comprehensive post-mortem analysis.
- Standardized protocols enhance the safety and efficacy of forensic investigations during pandemics.

