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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: Jul 9, 2025

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

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Single Genomic Loci Labeling and Manipulation Using SIMBA System.

Yanwei Chen^1,2, Ziliang Huang³, Qin Peng¹

¹Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, China.

Current Protocols

|December 6, 2023

Summary

This summary is machine-generated.

The SIMBA system uses CRISPR technology to simultaneously image and manipulate genomic loci in living cells. This innovative tool aids in studying genome organization and gene expression regulation.

Keywords:

CRISPR/Cas9 HP1α chromatin modeling live-cell imaging locus labeling

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Robust 3D DNA FISH Using Directly Labeled Probes

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

CRISPR-based technologies offer precise genomic targeting.
Visualizing and manipulating genomic loci in real-time is crucial for understanding cellular processes.
Existing methods may lack the ability for simultaneous imaging and manipulation.

Purpose of the Study:

To introduce the SIMBA (Simultaneous Imaging and Manipulation of genomic loci by Biomolecular Assemblies) system.
To demonstrate SIMBA's capability for simultaneous visualization and manipulation of genomic loci.
To provide protocols for utilizing SIMBA in various cell biology applications.

Main Methods:

The SIMBA system utilizes a CRISPR-based approach with dCas9-SunTag for targeting.
FRB-mCherry-HP1α fusion protein facilitates chromatin condensation at targeted loci.
A scFv-FKBP bridge protein links the components for efficient recruitment of HP1α.

Main Results:

SIMBA enables precise visualization of specific genomic sites.
The system allows for the condensation of chromatin at targeted loci.
SIMBA facilitates the study of genome organization, gene expression, and chromatin dynamics.

Conclusions:

The SIMBA system is an innovative tool for simultaneous imaging and manipulation of genomic loci.
This technique opens new avenues for research in genome organization and gene regulation.
SIMBA provides a robust platform for live-cell imaging and manipulation studies.