Antibacterial potentials of carbon dots immobilized on chitosan and glass surfaces

Due to their antibacterial activity, chitosan‑carbon dot composites possess great potential for pharmaceuticals, medicine, and food preservation. Conducting a comprehensive study of the interactions between chitosan, carbon dots, and bacteria is crucial to understanding the processes behind applying these composites. This study aimed to immobilize carbon dots (C-dots) synthesized from Elaeagnus angustifolia fruits on chitosan and glass microbeads' surfaces, to characterize the test materials obtained after synthesis and immobilization, and to investigate their antibacterial potentials. C-dot synthesis was carried out from water extract in an acidic medium with the help of microwave irradiation, and their structural and optical properties were characterized by TEM, XRD, FT-IR, UV-vis, Zeta potential, and fluorescence methods. The surface of the glass microbeads was first activated and functionalized with surface amine groups with a silaning agent. C-dots were immobilized on both glass and chitosan microbeads using a crosslinking agent. Antibacterial potentials of nine different test materials, obtained before or after immobilization, were evaluated both qualitatively (MIC and MBC) and quantitatively (GI₅₀) on E. coli, S. typhimurium, B. subtilis, and S. aureus, with the standard broth microdilution method. FT-IR and SEM-EDX analyses showed that C-dots were immobilized on chitosan (˂1 mm) and glass (˂100 μm) microbead surfaces. C-dots reduced the cell viability by ~25 % on S. typhimurium and B. subtilis (MIC = 25 mg/mL). It was also found that the highest antibacterial effect was recorded for C-dots-glass microbeads, which had a toxic effect of 43 % on S. aureus. In addition, binding C-dots to glass microbeads increased the antibacterial effect selectively in Gram-positive bacteria, while binding to chitosan microbeads was effective in all bacteria. The study showed that the antibacterial potential of C-dots-chitosan microbeads is more effective than C-dots-glass microbeads. C-dots could be used as carbon-based nanomaterials in antibacterial surface preparation once immobilized.