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Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

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Published on: October 25, 2018

Integrated Three-Dimensional Microdevice with a Modified Surface for Enhanced DNA Separation from Biological Samples.

Peipei Li¹, Menghang Li^1,2, Bing Sun²

¹Key Laboratory of Protection and Utilization of Aquatic Germplasm Resource, Ministry of Agriculture and Rural Affairs, Key Laboratory of Germplasm Improvement and Fine Seed Breeding for Marine Aquatic Animals, Liaoning Ocean and Fisheries Science Research Institute, Dalian, Liaoning 116023, China.

ACS Applied Materials & Interfaces

|December 7, 2023

Summary

3D printed microdevices functionalized with polydopamine (PDA) significantly enhance deoxyribonucleic acid (DNA) separation efficiency. These modified devices offer improved DNA extraction yields and selectivity for bioengineering applications.

Keywords:

3D printing DNA separation interfacial modification microscale devices nonmagnetic adsorption

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Area of Science:

Bioengineering
Materials Science
Biotechnology

Background:

Efficient nucleic acid (NA) adsorption and immobilization are crucial for bioengineering.
Existing methods require advanced functional interfaces and devices for rapid NA separation.

Purpose of the Study:

To develop and functionalize 3D printed microdevices for enhanced deoxyribonucleic acid (DNA) separation.
To investigate the impact of surface modifications on DNA binding affinity and extraction efficiency.

Main Methods:

Fabrication of poly(acrylic acid) (PAA) microdevices using 3D printing (D_PAA).
Surface modification of D_PAA with polydopamine (D_PDA-PAA) and poly(ethylene glycol) (D_PEG-PAA).
Characterization using surface potential analysis, molecular dynamics simulation, and DNA separation experiments.

Main Results:

D_PAA devices exhibited high printing accuracy (40-50 μm).
Surface functionalization with PDA and PEG was successful, maintaining thermal stability.
DNA affinity order was D_PDA-PAA > D_PEG-PAA > D_PAA, confirmed by experiments showing higher DNA extraction yield for D_PDA-PAA, especially in acidic conditions (pH 5.0-7.0).

Conclusions:

Surface modification of 3D printed microdevices effectively enhances DNA separation efficiency.
D_PDA-PAA demonstrates superior performance for DNA extraction, offering a nonmagnetic separation strategy.
This approach provides a new direction for designing advanced bioengineering separators and may benefit polymerase chain reaction-based diagnostics.