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Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
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B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

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The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
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Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

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The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
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Special Features of Adaptive Immunity01:20

Special Features of Adaptive Immunity

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The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
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Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

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Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
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T Cell Types and Functions01:24

T Cell Types and Functions

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When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
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Updated: Jul 9, 2025

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
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The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

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CD62L expression marks a functionally distinct subset of memory B cells.

Christopher H Hanson1, Brittany Henry1, Pradhnesh Andhare1

  • 1Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.

Cell Reports
|December 7, 2023
PubMed
Summary
This summary is machine-generated.

Memory B cell subsets are distinct and regulated by specific genes. CD62L expression identifies these functionally different populations in mice and humans after SARS-CoV-2 vaccination.

Keywords:
B cellsCP: Immunologyhumoral immunityimmunological memory

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The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
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Flow Cytometric Characterization of Murine B Cell Development
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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Memory B cell responses involve distinct subsets with varying antigen-reactivity.
  • Pathways governing memory B cell subset development and function remain largely unclear.

Purpose of the Study:

  • To investigate the regulatory mechanisms of memory B cell subset differentiation.
  • To identify markers that distinguish functionally distinct memory B cell subsets.

Main Methods:

  • Analysis of CD62L and CD44 expression on mouse memory B cells.
  • Investigating the role of Bcl6 and Zeb2 in B cell subset development.
  • Examining CD62L expression on human memory B cells post-SARS-CoV-2 vaccination.

Main Results:

  • CD62L and CD44 expression increases progressively on mouse memory B cells, defining distinct subsets.
  • Bcl6 is crucial for memory B cell subset differentiation; its overexpression impairs CD62L+ memory B cell development.
  • Bcl6 regulates genes like Bcl2 and Zeb2; Zeb2 overexpression also hinders CD62L+ memory B cell development.
  • CD62L is differentially expressed on human memory B cells after SARS-CoV-2 vaccination, identifying distinct populations.

Conclusions:

  • CD62L expression serves as a marker for functionally distinct memory B cell subsets.
  • These findings elucidate key regulators of memory B cell subset development and function.
  • CD62L's role in distinguishing memory B cell subsets is conserved across species and relevant in vaccine responses.