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Updated: Jul 9, 2025

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
Published on: February 28, 2019
High-Speed Atomic Force Microscopy for Filming Protein Molecules in Dynamic Action
Toshio Ando1, Shingo Fukuda1, Kien X Ngo1
1Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kanazawa, Japan;
None:
Structural biology is currently undergoing a transformation into dynamic structural biology, which reveals the dynamic structure of proteins during their functional activity to better elucidate how they function. Among the various approaches in dynamic structural biology, high-speed atomic force microscopy (HS-AFM) is unique in the ability to film individual molecules in dynamic action, although only topographical information is acquirable. This review provides a guide to the use of HS-AFM for biomolecular imaging and showcases several examples, as well as providing information on up-to-date progress in HS-AFM technology. Finally, we discuss the future prospects of HS-AFM in the context of dynamic structural biology in the upcoming era.
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