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Updated: Jul 9, 2025

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
Published on: February 8, 2020
Circ_0061140 Potentiates Clear Cell Renal Cell Carcinoma Progression Via the MicroRNA-126-5p/ADAM9 Axis
Guang Jia1, Dan Lv2, Shaobin Ni3
1Department of Urology Surgery, The First Affiliated Hospital of Harbin Medical University, No. 23, Youyou Street, Nangang District, Harbin, 150001, Heilongjiang, People's Republic of China. 813747@hrbmu.edu.cn.
Abstract:
Circular RNAs (circRNAs) function as new cancer biomarkers, but the role of circ_0061140 remains unknown in clear cell renal cell carcinoma (ccRCC). Therefore, we aimed to validate the functions of circ_0061140 in ccRCC and its potential as a prognostic biomarker. At first, circ_0061140 expression in ccRCC tissues and cells was detected, and circ_0061140 was upregulated in ccRCC tissues (p < 0.0001) and cells (p < 0.0001). Patients with high expression of circ_0061140 had a worse prognosis (p < 0.05). Then, siRNA against circ_0061140 was transfected into Caki-1 and UT14 cells to explore its roles in the biological functions of ccRCC cells, and suppressing roles of downregulated circ_0061140 were observed in the cell growth of Caki-1 and UT14 cells (p < 0.01). Next, circ_0061140 was found to be a sponge of miR-126-5p, and ADAM9 was determined to be a target of miR-126-5p. Finally, functional rescue experiments were conducted to observe their roles in ccRCC cell growth. It was suggested that suppressed miR-126-5p or overexpressed ADAM9 induced cell proliferation and restricted cell apoptosis in ccRCC cells based on si-circ_0061140 (p < 0.01). Altogether, this study highlights that circ_0061140 plays an oncogenic role in ccRCC through modulation of the miR-126-5p/ADAM9 axis.
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