Multiple Non-Covalent Cross-Linked Multifunctional Strong Hemostatic Agent for Dynamic Exposure Hemostasis

Area of Science:

• Biomaterials Science
• Trauma Care
• Hemostasis

Background:

• Effective trauma management requires immediate and durable hemostasis.
• Current hemostatic agents face limitations in mechanical strength and storage stability.
• Achieving rapid hemostasis with good mechanical properties, stability, and biocompatibility remains a significant challenge.

Purpose of the Study:

• To develop and characterize a novel hemostatic material with enhanced properties for trauma applications.
• To address the limitations of existing hemostatic materials in terms of mechanical strength, storage, and efficacy.

Main Methods:

• Development of a novel hemostatic material utilizing multiple non-covalent bond crosslinking.
• Characterization of hydrogel micro-modules (HM) for rapid gelation upon exposure to saline.
• Evaluation of mechanical properties (tensile strength, elongation at break), storage stability, biocompatibility (cell viability), and hemolysis rate.
• Assessment of hemostatic efficacy in dynamic experiments simulating transportation to prevent secondary bleeding.

Main Results:

• The novel HM rapidly forms a stable gel barrier within 20 seconds in physiological saline.
• The resulting gel exhibits high tensile strength (62.10 kPa) and significant elongation at break (1976%).
• The material maintains its mechanical properties after 30 days of storage.
• High cell viability (>80%) and a low hemolysis rate (2.08%) indicate good biocompatibility.
• Effective prevention of secondary bleeding in dynamic hemostasis simulations was demonstrated.

Conclusions:

• The developed hemostatic material, based on non-covalent crosslinking, offers a promising solution for trauma care.
• It provides rapid hemostasis, excellent mechanical properties, and superior storage stability.
• This novel biomaterial exhibits favorable biocompatibility and effectively prevents re-bleeding in dynamic conditions, indicating its potential for practical applications.