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Updated: Jul 9, 2025

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants
Published on: June 9, 2022
Reverse Genetics Systems for Filoviruses
Bianca S Bodmer1, Thomas Hoenen2
1Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Greifswald, Insel Riems, Germany.
Abstract:
Filoviruses are causative agents of severe hemorrhagic fevers with high case fatality rates in humans. For studies of virus biology and the subsequent development of countermeasures, reverse genetic systems, and especially those facilitating the generation of recombinant filoviruses, are indispensable. Here, we describe the generation of recombinant filoviruses from cDNA.
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