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Quantifying nanoparticle delivery: challenges, tools, and advances.

Mario Y Mata Corral1, Damian E Alvarez1, Wilson Poon1

  • 1Department of Metallurgical, Materials, and Biomedical Engineering, University of Texas at El Paso, 500 W University Ave, El Paso, TX 79968, USA.

Current Opinion in Biotechnology
|December 8, 2023
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Summary
This summary is machine-generated.

Quantifying nanoparticle delivery in therapies is complex. This review highlights challenges and methods, emphasizing the need for careful selection and integration of techniques for accurate in vivo tracking and clinical translation.

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Area of Science:

  • Biomedical Engineering
  • Nanomedicine
  • Drug Delivery Systems

Background:

  • Accurate quantification of nanoparticle delivery is crucial for therapeutic efficacy.
  • Current methods for tracking nanoparticles in vivo vary significantly.
  • Inconsistent quantification can lead to misleading conclusions in nanomedicine research.

Purpose of the Study:

  • To review existing methods for quantifying nanoparticle delivery in therapeutic applications.
  • To identify challenges and potential pitfalls associated with different quantification approaches.
  • To highlight advancements in novel technologies for nanoparticle tracking.

Main Methods:

  • Exploration of three primary quantification strategies: functional readouts, nanocarrier tracking, and cargo tracking.
  • Reanalysis of the Wilhelm et al. Cancer Nanomedicine Repository dataset to assess quantification methodologies.
  • Discussion of potential limitations and recent technological innovations.

Main Results:

  • Identified mixed quantification methodologies in existing datasets, potentially causing misleading conclusions.
  • Detailed the pitfalls associated with functional readouts, nanocarrier tracking, and cargo tracking.
  • Highlighted emerging technologies for improved nanoparticle localization assessment.

Conclusions:

  • Researchers must select appropriate quantification methods based on specific objectives.
  • Integrating multiple quantification approaches offers a comprehensive understanding of in vivo nanoparticle behavior.
  • Improved quantification is essential for facilitating the clinical translation of nanoparticle-based therapies.