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Updated: Jul 5, 2026

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Published on: January 7, 2019

Selecting Suitable Near-Native Lignins for Research.

Mingjie Chen¹, John Ralph^2,3, Jeremy S Luterbacher⁴

¹Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, People's Republic of China.

Journal of Agricultural and Food Chemistry

|December 8, 2023

Summary

Enzymatic lignin or cellulolytic enzyme lignin best represent native lignin in softwoods and hardwoods. However, these methods do not accurately represent grass lignin due to syringyl/guaiacyl differences and interfering hydroxycinnamates.

Keywords:

cellulolytic enzyme lignin enzymatic lignin enzymatic mild-acidolysis lignin milled-wood lignin native lignin

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Last Updated: Jul 5, 2026

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Quantitative 31P NMR Analysis of Lignins and Tannins

Quantitative 31P NMR Analysis of Lignins and Tannins

Published on: August 2, 2021

Area of Science:

Biomass Science
Polymer Chemistry
Plant Biology

Background:

Accurate characterization of native lignin is crucial for biomass utilization.
Several methods exist for isolating near-native lignin, but their representativeness varies.
Understanding lignin structure is key to unlocking its potential in various applications.

Purpose of the Study:

To evaluate the representativeness of different near-native lignin isolation methods.
To compare the structural characteristics of isolated lignins with their native counterparts across diverse plant groups.
To identify the most suitable lignin isolation methods for softwoods, hardwoods, and grasses.

Main Methods:

Isolation of near-native lignins using milled-wood lignin, enzymatic lignin, cellulolytic enzyme lignin, and enzymatic mild-acidolysis lignin methods.
Structural analysis including molecular weight determination, monomer composition, and interunit linkage distribution.
Comparative analysis of isolated lignins from different plant groups (softwoods, hardwoods, grasses).

Main Results:

Enzymatic lignin and cellulolytic enzyme lignin were found to be representative of native lignins in softwoods and hardwoods.
None of the tested methods adequately represented native lignin in grasses due to significant syringyl/guaiacyl variations.
Hydroxycinnamates in grasses interfere with lignin analysis, complicating structural elucidation.

Conclusions:

Enzymatic and cellulolytic enzyme lignin isolation methods are suitable for softwoods and hardwoods.
Current near-native lignin isolation techniques are insufficient for accurately representing grass lignin structure.
Further research is needed to develop methods for accurate grass lignin analysis, considering the role of hydroxycinnamates.