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Machine-learning guided Venom Induced Dermonecrosis Analysis tooL: VIDAL.

William Laprade1, Keirah E Bartlett2, Charlotte R Christensen3

  • 1Department of Applied Mathematics and Computer Science, Technical University of Denmark, Kongens Lyngby, Denmark.

Scientific Reports
|December 8, 2023
PubMed
Summary
This summary is machine-generated.

Snakebite envenoming causes severe tissue damage. A new AI tool, VIDAL, accurately measures necrosis in mice, improving preclinical antivenom testing for this neglected tropical disease.

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Area of Science:

  • Toxicology
  • Biomedical Engineering
  • Machine Learning

Background:

  • Snakebite envenoming is a major global health concern, causing significant mortality and morbidity, especially in low-income countries.
  • Clinical effects include paralysis, hemorrhage, and necrosis, necessitating effective antivenom therapies.
  • Preclinical assessment of antivenoms against dermonecrosis in mouse models is crucial but current methods are labor-intensive and error-prone.

Purpose of the Study:

  • To develop and validate an automated, machine-learning-based tool for quantifying dermonecrosis in mouse models.
  • To introduce a novel metric, the dermonecrotic unit (DnU), for a more comprehensive assessment of necrosis severity.
  • To provide a high-throughput, accurate, and reproducible method for evaluating antivenom efficacy against cytotoxic snakebite effects.

Main Methods:

  • Development of the Venom Induced Dermonecrosis Analysis tooL (VIDAL), an image-based machine learning solution.
  • VIDAL automatically identifies dermonecrotic lesions, corrects for lighting variations, scales images, and extracts lesion area and discoloration data.
  • Calculation of dermonecrosis severity using the newly defined dermonecrotic unit (DnU).

Main Results:

  • VIDAL accurately quantifies dermonecrotic lesions in mouse models.
  • The tool's performance is comparable to traditional histopathological analysis.
  • The dermonecrotic unit (DnU) provides a more nuanced measure of necrosis severity.

Conclusions:

  • VIDAL offers an accessible, accurate, and reproducible method for assessing dermonecrosis in preclinical snakebite research.
  • This high-throughput technology is vital for accelerating the development and validation of snakebite therapeutics.
  • Automated analysis tools like VIDAL are essential for addressing snakebite envenoming, a neglected tropical disease.