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Updated: Jul 9, 2025

Chromatin Immunoprecipitation ChIP to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
Published on: July 29, 2010
Chromatin Remodeling Complex PBAF Activates and Represses Inflammatory Genes
A V Feoktistov1,2, S G Georgieva3, N V Soshnikova3,4
1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. a.feo95@mail.ru.
Abstract:
The PBAF chromatin remodeling complex regulates chromatin state and gene transcription in higher eukaryotes. In this work, we studied the role of PBAF in the regulation of NF-κB-and JAK/STAT-dependent activation of inflammatory genes. We performed knockdown of specific module subunit BAF200, which resulted in destruction of the entire PBAF specific module and changed the level of the genes transcription of both pathways. PBAF can be both an activator and a repressor of inflammatory genes. Thus, PBAF is an important regulator of inflammatory gene expression.
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