Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

70.7K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.7K
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

62.2K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
62.2K
Bacterial Signaling01:30

Bacterial Signaling

32.3K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
32.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A genomically unique lytic bacteriophage infecting Pseudomonas syringae pv. actinidiae.

Virology·2026
Same author

Evaluation of the delivery of an anti-Listeria endolysin via CRISPR-Cas9 engineered probiotic Saccharomyces boulardii.

Applied microbiology and biotechnology·2026
Same author

Microneedle-integrated biosensors for smart wound monitoring.

Biosensors & bioelectronics·2026
Same author

Phage-loaded alginate films and coatings for biofilm inhibition and control in food packaging.

Food research international (Ottawa, Ont.)·2025
Same author

Directed evolution of phages in biofilms enhances Pseudomonas aeruginosa control through improved lipopolysaccharide recognition.

Nature communications·2025
Same author

Lytic properties and genomic analysis of bacteriophage Brt_Psa3, targeting Pseudomonas syringae pv. actinidiae.

Applied microbiology and biotechnology·2025

Related Experiment Video

Updated: Jul 9, 2025

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings
07:22

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

Published on: August 19, 2021

3.0K

Bacteriophage Control of Infectious Biofilms.

Luciana Meneses1,2, Sanna Sillankorva3, Joana Azeredo4,5

  • 1CEB - Centre of Biological Engineering, University of Minho, Braga, Portugal.

Methods in Molecular Biology (Clifton, N.J.)
|December 8, 2023
PubMed
Summary

Bacteriophages show promise for controlling bacterial biofilms, which are difficult to treat with antibiotics. This chapter outlines methods to study bacteriophage efficacy against biofilms, from formation to treatment evaluation.

Keywords:
BacteriophageBiofilmControlQuantification

More Related Videos

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides
07:19

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides

Published on: June 28, 2024

942
Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors
10:17

Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors

Published on: October 9, 2016

15.2K

Related Experiment Videos

Last Updated: Jul 9, 2025

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings
07:22

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

Published on: August 19, 2021

3.0K
Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides
07:19

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides

Published on: June 28, 2024

942
Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors
10:17

Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors

Published on: October 9, 2016

15.2K

Area of Science:

  • Microbiology
  • Bacteriophage research
  • Antimicrobial strategies

Background:

  • Bacterial biofilms pose significant challenges in healthcare due to their resistance to conventional antimicrobial therapies.
  • Infectious biofilms are a major concern, necessitating novel approaches for prevention and treatment.
  • Bacteriophages, viruses that infect bacteria, offer a potential alternative for combating biofilm-related infections.

Purpose of the Study:

  • To describe standard procedures for evaluating bacteriophages as agents for biofilm control.
  • To provide a framework for studying bacteriophage efficacy in preventing and eradicating bacterial biofilms.
  • To facilitate research into phage therapy for biofilm-associated infections.

Main Methods:

  • Standardized protocols for inducing and monitoring bacterial biofilm formation.
  • Methods for applying bacteriophage treatments to established biofilms.
  • Techniques for quantifying biofilm biomass and bacterial viability post-treatment.
  • Assays to evaluate the efficacy of bacteriophages in reducing or eliminating biofilms.

Main Results:

  • The described procedures allow for systematic assessment of bacteriophage activity against biofilms.
  • Standardized methods enable reproducible evaluation of phage therapeutic potential.
  • The study provides a basis for comparing different bacteriophages and treatment strategies.

Conclusions:

  • Bacteriophages are a viable and promising strategy for controlling bacterial biofilms.
  • Standardized methodologies are crucial for advancing bacteriophage-based biofilm control research.
  • Further investigation using these procedures can lead to effective phage therapies against biofilm infections.