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Updated: Jul 9, 2025

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
Published on: September 3, 2013
Anomalous, dielectrophoretic transport of molecules in non-electrolytes
Gaurav Anand1, Samira Safaripour1, Craig Snoeyink1
1Department of Mechanical and Aerospace Engineering, University at Buffalo, Buffalo, New York, USA.
Abstract:
The electric field (E-field) dielectric polarization-based separations mechanism represents a novel method for separating solutions at small length scales. An E-field gradient with a maximum strength of 0.4 MV/m applied across a 10 μm deep channel is shown to increase the concentration inside the low E-field region by ≈ 40% relative to the high E-field region. This concentration change is two orders of magnitude higher than the estimated change predicted using the classical equilibrium thermodynamics for the same E-field. The deviation between the predicted and the experimental results suggests that the change in volumetric E-field energy with solute concentration is insufficient to explain this phenomenon. The study also explores the effect of varying strength of E-field and frequency of supplied voltage on the dielectric polarization-based separation efficiency. While the increase in the former increases the separation efficiency, the increase in the latter reduces the degree of concentration change due to ineffective charging of the electrodes.
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