Secretome Analysis of High- and Low-Virulent Bovine Pasteurella multocida Cultured in Different Media
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Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
Published on: February 22, 2017
Yangyang Qiu1, Jianan Wang1, Fang He1
1College of Veterinary Medicine, Southwest University, Chongqing 400715, China.
Abstract:
Bovine Pasteurella multocida (P. multocida) serotype A is one of the major causes of bovine respiratory disease (BRD). We used data-independent acquisition (DIA) LC-MS/MS combined with bioinformatics analysis to identify proteins secreted by P. multocida. A total of 154 proteins were obtained from the supernatants of two isolates of bovine P. multocida serotype A (high virulent PmCQ2 and low virulent PmCQ6) cultured in Martin or BHI media, of which 50 were identified as putative secreted proteins. Further studies showed that Tuf, an elongation factor Tu, was highly expressed in P. multocida and secreted into infected tissues. Tuf stimulated strong innate immune responses of macrophages and had protective efficacy against P. multocida infection in a mouse model. The results provide insight into the secreted proteins of P. multocida and suggest new targets for vaccine development against P. multocida.
Insights
Bovine respiratory disease (BRD) is often caused by Pasteurella multocida. Researchers identified secreted proteins, including Tuf, which shows potential for developing new vaccines against P. multocida infections.
Area of Science:
- Veterinary Microbiology
- Proteomics
- Immunology
Background:
- Bovine respiratory disease (BRD) is a significant concern in cattle health.
- Bovine Pasteurella multocida (P. multocida) serotype A is a primary etiological agent of BRD.
- Understanding secreted proteins is crucial for developing effective control strategies.
Purpose of the Study:
- To identify and characterize proteins secreted by bovine P. multocida serotype A.
- To investigate the role of identified secreted proteins in host-pathogen interactions.
- To explore potential vaccine targets against P. multocida.
Main Methods:
- Data-independent acquisition (DIA) LC-MS/MS was employed for proteomic analysis.
- Bioinformatics tools were utilized to identify secreted proteins from P. multocida isolates.
- In vitro macrophage assays and a mouse infection model were used to assess protein function and efficacy.
Main Results:
- A total of 154 proteins were identified, with 50 designated as putative secreted proteins.
- Tuf (elongation factor Tu) was found to be highly expressed and secreted by P. multocida.
- Tuf stimulated innate immune responses and demonstrated protective efficacy in a mouse model.
Conclusions:
- The study provides a comprehensive list of secreted proteins from bovine P. multocida.
- Tuf is identified as a key secreted protein with immunomodulatory and protective properties.
- These findings suggest Tuf as a promising candidate for novel vaccine development against BRD.
