Cytotoxic Natural Products Isolated from Cryptogramma crispa (L.) R. Br

Area of Science:

• Phytochemistry
• Natural Product Chemistry
• Pharmacology

Background:

• Parsley fern (Cryptogramma crispa) is an arctic-alpine plant with a history of presumed toxicity but uninvestigated natural products.
• Traditional knowledge suggests potential medicinal or toxic properties, necessitating scientific validation.

Purpose of the Study:

• To characterize the natural products of Cryptogramma crispa.
• To evaluate the cytotoxic activity of isolated compounds against cancer cell lines.

Main Methods:

• Isolation and characterization of 15 natural products from aerial parts.
• Structure elucidation using advanced NMR, Circular Dichroism, and high-resolution mass spectrometry.
• In vitro cytotoxicity assays against acute myeloid leukemia (MOLM13) and normal cell lines.

Main Results:

• 15 natural products were identified, including a new compound, 3-malonyl pteroside D.
• Pteroside derivatives demonstrated selective moderate cytotoxicity against MOLM13 cells.
• No significant cytotoxicity was observed against normal heart and kidney cells.

Conclusions:

• Cryptogramma crispa contains novel pteroside derivatives with selective anticancer potential.
• Further investigation into the anticancer effects of these compounds is warranted.
• This research opens avenues for developing new leukemia therapies from natural sources.