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Cellulose and Pectic Polysaccharides01:15

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 Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth.  Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...
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Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
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Polysaccharides from Radix Peucedani: Extraction, Structural Characterization and Antioxidant Activity.

Jie Zhang1, Chenyue Wang1, Qian Li1

  • 1State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.

Molecules (Basel, Switzerland)
|December 9, 2023
PubMed
Summary

Ultrasound-assisted green extraction using deep eutectic solvents (DESs) efficiently yielded polysaccharides from Radix Peucedani. These polysaccharides demonstrated antioxidant activity, potentially through interactions with specific proteins like IL1B and CASP3.

Keywords:
Radix Peucedaniantioxidantdeep eutectic solventsmolecular dockingnetwork pharmacologypolysaccharides

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Area of Science:

  • Natural Product Chemistry
  • Green Extraction Technologies
  • Pharmacology

Background:

  • Radix Peucedani is a traditional medicinal herb with potential health benefits.
  • Efficient extraction of bioactive compounds like polysaccharides is crucial for their utilization.
  • Deep eutectic solvents (DESs) offer a sustainable alternative for green extraction processes.

Purpose of the Study:

  • To develop and optimize an ultrasound-assisted green extraction method for polysaccharides from Radix Peucedani using DESs.
  • To characterize the physicochemical properties and evaluate the in vitro antioxidant activities of the extracted polysaccharides.
  • To elucidate the potential antioxidant mechanism of Radix Peucedani polysaccharides using network pharmacology and molecular docking.

Main Methods:

  • Ultrasound-assisted extraction using optimized DES (betaine and 1,2-propylene glycol, 1:2 molar ratio).
  • Response surface methodology (Box-Behnken design) to optimize extraction parameters (DES water content, time, temperature, solid-liquid ratio, ultrasonic power).
  • Physicochemical analysis (ICS, FT-IR, SEM) and in vitro antioxidant assays (DPPH, Hydroxyl, ABTS+).
  • Network pharmacology and molecular docking for mechanism investigation.

Main Results:

  • Optimal extraction conditions yielded 11.372% polysaccharides.
  • Physicochemical analysis confirmed the properties of the extracted polysaccharides.
  • Significant in vitro antioxidant activities (DPPH, Hydroxyl, ABTS+ radical scavenging) were observed.
  • Network pharmacology and molecular docking suggested potential antioxidant mechanisms involving glucose-IL1B and galactose-CASP3 interactions.

Conclusions:

  • Ultrasound-assisted green extraction with optimized DES is an effective method for obtaining Radix Peucedani polysaccharides.
  • The extracted polysaccharides possess notable antioxidant properties.
  • Potential antioxidant mechanisms involve specific molecular interactions identified through computational methods.