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Resistomycin Suppresses Prostate Cancer Cell Growth by Instigating Oxidative Stress, Mitochondrial Apoptosis, and

Abeer S Aloufi1, Ola A Habotta2, Mohamed S Abdelfattah3

  • 1Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.

Molecules (Basel, Switzerland)
|December 9, 2023
PubMed

Insights

Resistomycin, a marine-derived antibiotic, shows promise as a prostate cancer treatment by inducing cell death and halting cell cycle progression. This natural compound offers a potential new avenue for chemotherapy with fewer side effects.

Area of Science:

  • Marine natural products
  • Cancer biology
  • Drug discovery

Background:

  • Prostate cancer is a leading cause of male mortality worldwide.
  • Current treatments face challenges with efficacy, drug resistance, and adverse effects.
  • Resistomycin, a marine actinomycete antibiotic, exhibits diverse biological activities.

Purpose of the Study:

  • To evaluate resistomycin's potential as an antitumor agent against prostate cancer.
  • To investigate its mechanism of action, including effects on cell viability, apoptosis, and cell cycle.
  • To compare its efficacy with a standard chemotherapy drug, 5-fluorouracil (5-FU).

Main Methods:

  • Prostate cancer (PC3) cells were treated with varying concentrations of resistomycin or 5-FU.
  • Cell viability and apoptosis were assessed using MTT assay and flow cytometry.
  • Oxidative stress markers, apoptotic markers, and cell cycle regulators were analyzed.

Main Results:

  • Resistomycin demonstrated significant cytotoxicity against PC3 cells (IC50: 2.63 µg/mL) compared to 5-FU (IC50: 14.44 µg/mL).
  • Resistomycin induced apoptosis, increased reactive oxygen species, and altered levels of key apoptotic and cell cycle proteins in a dose-dependent manner.
  • The compound triggered oxidative stress and mitochondrial apoptosis pathways, leading to cell cycle arrest.

Conclusions:

  • Resistomycin exhibits potent antitumor activity against prostate cancer cells.
  • Its mechanism involves inducing oxidative stress, mitochondrial apoptosis, and cell cycle arrest.
  • Resistomycin represents a promising lead compound for developing novel prostate cancer chemotherapeutics.
Keywords:
5-FUapoptosisoxidative stressprostate cancerresistomycin

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