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Related Concept Videos

Mesenchymal Stem Cells01:19

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
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Human Umbilical Cord Mesenchymal-Stem-Cell-Derived Extracellular Vesicles Reduce Skin Inflammation In Vitro.

Tzou-Yien Lin1, Tsong-Min Chang2, Wei-Cheng Tsai3

  • 1Department of Paediatrics, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan 33305, Taiwan.

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|December 9, 2023
PubMed
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Human umbilical cord mesenchymal stem cell-derived extracellular vesicles (UCMSC-EVs) protect skin cells from damage. These EVs boost antioxidant defenses and reduce inflammation, showing potential for anti-aging skincare products.

Keywords:
NF-κBSIRT1extracellular vesiclep53umbilical-cord-derived mesenchymal stem cells

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Area of Science:

  • Cell Biology
  • Dermatology
  • Regenerative Medicine

Background:

  • Oxazolone induces damage in human keratinocytes (HaCaT cells), mimicking skin injury.
  • Extracellular vesicles (EVs) from mesenchymal stem cells are investigated for therapeutic potential.
  • Human umbilical cord mesenchymal stem cells (UCMSC) are a source of potent EVs.

Purpose of the Study:

  • To investigate the protective effects of UCMSC-derived EVs against oxazolone-induced damage in HaCaT cells.
  • To explore the underlying mechanisms of UCMSC-EVs' protective and anti-inflammatory actions.
  • To assess the potential of UCMSC-EVs in anti-aging cosmetic applications.

Main Methods:

  • HaCaT cells were pretreated with UCMSC-EVs before oxazolone exposure.
  • Cell viability, reactive oxygen species (ROS) levels, and mitochondrial membrane potential were measured.
  • Inflammatory cytokines (IL-1β, TNF-α), SIRT1, P53, and P65 protein expressions were analyzed.

Main Results:

  • UCMSC-EV pretreatment significantly improved cell viability and reduced ROS and mitochondrial damage.
  • UCMSC-EVs demonstrated anti-inflammatory effects by decreasing IL-1β and TNF-α levels.
  • Mechanism analysis revealed increased SIRT1 and P53, and decreased P65 protein expression.

Conclusions:

  • UCMSC-derived EVs possess protective properties against oxazolone-induced skin cell damage.
  • UCMSC-EVs enhance cellular antioxidant defense systems and exhibit anti-inflammatory activity.
  • These findings suggest UCMSC-EVs hold promise as functional ingredients for anti-aging skincare.