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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
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Related Experiment Video

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Challenges in ESI-MS-based Untargeted Metabolomics.

Elena Tobolkina1,2, Víctor González-Ruiz3,2,4, Isabel Meister5,2

  • 1School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland. Elena.Tobolkina@unige.ch.

Chimia
|December 9, 2023
PubMed
Summary
This summary is machine-generated.

This review overviews liquid-phase separation methods coupled with mass spectrometry (MS) for untargeted metabolomics. It offers practical guidance on data acquisition, processing, and interpretation for biological samples.

Keywords:
AnnotationCapillary electrophoresisLiquid chromatographyMass spectrometryMetabolomicsSupercritical fluid chromatographyToxicological and doping analysis

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Systems Biology

Background:

  • Untargeted metabolomics is crucial for understanding biological system responses.
  • Liquid-phase separation techniques coupled with mass spectrometry are key for comprehensive metabolic profiling.

Purpose of the Study:

  • To provide an overview of liquid-phase separation methods for untargeted metabolomics.
  • To offer practical guidance on data acquisition, processing, and interpretation.
  • To highlight common challenges and solutions in metabolomic studies.

Main Methods:

  • Liquid chromatography-mass spectrometry (LC-MS) for untargeted metabolomics.
  • Gas chromatography-mass spectrometry (GC-MS) for untargeted metabolomics.
  • Capillary electrophoresis-mass spectrometry (CE-MS) for untargeted metabolomics.

Main Results:

  • Detailed review of various liquid-phase separation techniques applicable to untargeted metabolomics.
  • Discussion on data acquisition strategies to maximize metabolite coverage.
  • Insights into signal processing, data treatment, and biological interpretation.

Conclusions:

  • Liquid-phase separation hyphenated to MS is a powerful approach for untargeted metabolomics.
  • Careful consideration of methods and data handling is essential for reliable results.
  • This review serves as a practical guide for researchers in the field.