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Updated: Jul 9, 2025
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Production of Human CRISPR-Engineered CAR-T Cells
Published on: March 15, 2021
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Transposon-Based Manufacturing of Human CAR-T Cells
Megan Tennant1, Richard O'Neil2,3
1Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 9, 2023
Abstract:
In this chapter, the methodologies are outlined for generating CAR-T from PBMCs using transposon engineering. Additionally, some methods and guidance related to basic functional and phenotypic analysis are described. This methodology can be applied to manufacture and assess chimeric antigen receptors for preclinical applications targeting a variety of molecules.
