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Related Experiment Video

Updated: Jul 9, 2025

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
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Loop-mediated isothermal amplification coupled with nanoparticle-based lateral flow biosensor for monkeypox virus

Fei Xiao1, Jin Fu1, Xiaolan Huang1

  • 1Experimental research center, Capital Institute of pediatrics, Beijing, 100020, PR China.

Talanta
|December 9, 2023
PubMed
Summary

A new Monkeypox virus (MPXV) diagnostic test, MPXV-LAMP-LFB, offers rapid, sensitive, and specific detection. This molecular tool aids in early MPXV diagnosis and clade differentiation within an hour using simple equipment.

Keywords:
Lateral flow biosensorLoop-mediated isothermal amplificationMPXV infectionMonkeypox virusNanoparticle

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Area of Science:

  • Virology
  • Molecular Diagnostics
  • Biotechnology

Background:

  • Monkeypox virus (MPXV) poses a growing public health challenge.
  • Accurate and rapid MPXV detection is crucial for effective disease management.
  • Existing diagnostic methods may lack speed or accessibility.

Purpose of the Study:

  • To develop a novel diagnostic assay for Monkeypox virus detection.
  • To achieve rapid, sensitive, and specific identification of MPXV and its clades.
  • To create a user-friendly test requiring minimal specialized equipment.

Main Methods:

  • Development of MPXV-LAMP-LFB assay combining loop-mediated isothermal amplification (LAMP) and lateral flow biosensor (LFB).
  • Design of specific LAMP primers targeting MPXV D14L and ATI genes for clade differentiation.
  • Optimization of reaction conditions for rapid DNA extraction, amplification, and visual detection.

Main Results:

  • The MPXV-LAMP-LFB assay demonstrated high sensitivity, detecting as low as 5 copies of plasmid DNA and 12.5 copies of pseudotyped virus.
  • The test accurately identified MPXV positive samples and showed no cross-reactivity with other pathogens, confirming high specificity.
  • The entire diagnostic process, including sample preparation, can be completed within approximately one hour.

Conclusions:

  • The MPXV-LAMP-LFB assay is a promising molecular tool for the rapid and accurate diagnosis of Monkeypox virus infection.
  • Its simplicity and visual readout make it suitable for diverse settings, potentially aiding in outbreak response.
  • The assay facilitates differentiation between MPXV clades, crucial for epidemiological surveillance.