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Sr65: a widely effective gene for stem rust resistance in wheat
Michael Norman1,2, Chunhong Chen2, Hanif Miah1
1Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, 107 Cobbitty Road, Cobbitty, NSW, 2570, Australia.
A novel stem rust resistance gene, Sr65, was identified in Indian wheat landrace Hango-2. This gene, located on chromosome 1A, provides protection against Puccinia graminis f. sp. tritici races globally.
Area of Science:
- Plant genetics
- Crop science
- Molecular biology
Background:
- Stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), poses a significant threat to global wheat production.
- Virulent Pgt races like TTKSK and TTRTF have emerged, necessitating new resistance strategies.
- Indian wheat landrace Hango-2 exhibits resistance to Pgt races prevalent in India and Australia.
Purpose of the Study:
- To identify and map novel stem rust resistance genes in wheat.
- To characterize the genetic basis of resistance in the Indian landrace Hango-2.
- To develop molecular markers for marker-assisted selection of stem rust resistance.
Main Methods:
- Screening of a Hango-2/Avocet 'S' recombinant inbred line population.
- Genetic mapping of the resistance gene SrH2 using KASP markers.
- MutRenSeq and comparative genomic analysis.
- Development of a Hango-2 specific assembly for gene identification.
Main Results:
- Two stem rust resistance genes were identified: SrH2 (novel) and Sr26.
- SrH2 was mapped to chromosome arm 1AS, delimited to a 1.8-2.3 Mb interval.
- SrH2-linked KASP markers (KASP_12147 and sunCS_265) were identified with high polymorphism rates.
- SrH2 represents a new stem rust resistance locus, designated Sr65.
Conclusions:
- Sr65, located on chromosome 1A, is a valuable all-stage resistance gene effective against diverse Pgt races.
- The identified markers facilitate marker-assisted selection for Sr65 in wheat breeding programs.
- Sr65 is a crucial genetic resource for developing durable stem rust-resistant wheat cultivars worldwide.
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