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Rab11 suppresses head and neck carcinoma by regulating EGFR and EpCAM exosome secretion

Kunihiro Yoshida1, Kaung Htike2, Takanori Eguchi3

  • 1Department of Dental Pharmacology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan; Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8525, Japan.

Journal of Oral Biosciences
|December 10, 2023
PubMed
Abstract

Insights

Rab11 proteins suppress head and neck squamous cell carcinoma (HNSCC) progression by regulating receptor recycling and exosome secretion. Rab11b is a promising prognostic marker for HNSCC patients.

Area of Science:

  • Cell Biology
  • Cancer Research
  • Molecular Biology

Background:

  • Rab11 proteins (Rab11a and Rab11b) are key regulators of intracellular trafficking, involved in receptor recycling and exosome secretion.
  • While Rab11's role in cancer progression is known, its specific function in head and neck squamous cell carcinoma (HNSCC) remains largely uncharacterized.

Purpose of the Study:

  • To investigate the roles of Rab11a and Rab11b in HNSCC.
  • To determine the clinical significance of Rab11 expression in HNSCC patients.

Main Methods:

  • Utilized public database analysis and tissue microarray to assess Rab11 expression in HNSCC.
  • Generated stable HNSCC cell lines with altered Rab11a or Rab11b expression (loss and gain) to study cellular functions.
  • Assessed effects on proliferation, migration, invasion, epidermal growth factor receptor (EGFR) recycling, and exosome secretion.

Main Results:

  • Rab11b mRNA expression correlated with favorable patient survival in HNSCC.
  • Rab11b expression decreased with advancing HNSCC stages.
  • Overexpression of Rab11a/Rab11b reduced EGFR, EpCAM exosome secretion, migration, and invasion.
  • Knockdown of Rab11a/Rab11b increased EpCAM/CD9 exosome secretion, migration, and invasion.

Conclusions:

  • Rab11 suppresses HNSCC progression by modulating EGFR recycling and EpCAM exosome secretion.
  • Rab11b serves as a significant prognostic indicator for HNSCC.
  • Rab11 proteins represent potential therapeutic targets for HNSCC.
Keywords:
Epidermal growth factor receptorExosomeExtracellular vesicleHead and neck squamous cell carcinomaRab11

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