Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pulmonary fibroblasts: a model system for studying elastin synthesis.

R Campagnone, J Regan, C B Rich

    Laboratory Investigation; a Journal of Technical Methods and Pathology
    |February 1, 1987
    PubMed
    Summary

    Neonatal rat lung fibroblasts synthesize tropoelastin, forming insoluble elastin over 4 weeks. This study establishes a model for investigating lung interstitial elastin synthesis regulation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The effect of a postbiotic produced by stabilized non-viable Lactobacilli on the health, growth performance, immunity, and gut status of colisepticaemic broiler chickens.

    Tropical animal health and production·2022
    Same author

    Immune tolerance of allogeneic haematopoietic cell transplantation supports donor epidermal grafting of recessive dystrophic epidermolysis bullosa chronic wounds.

    The British journal of dermatology·2020
    Same author

    Revertant mosaic fibroblasts in recessive dystrophic epidermolysis bullosa.

    The British journal of dermatology·2019
    Same author

    Bone marrow transplant with post-transplant cyclophosphamide for recessive dystrophic epidermolysis bullosa expands the related donor pool and permits tolerance of nonhaematopoietic cellular grafts.

    The British journal of dermatology·2019
    Same author

    Genome-wide association studies of placebo and duloxetine response in major depressive disorder.

    The pharmacogenomics journal·2017
    Same author

    Gut Microbiome and Behavior: Focus on Neuroimmune Interactions.

    International review of neurobiology·2016

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Pulmonary Medicine

    Background:

    • Pulmonary interstitial fibroblasts are crucial for lung development and function.
    • Elastin synthesis and deposition are key processes in lung tissue formation and maintenance.
    • Understanding fibroblast behavior in the lung interstitium is vital for respiratory health research.

    Purpose of the Study:

    • To investigate the capacity of neonatal rat pulmonary interstitial fibroblasts to synthesize soluble and insoluble elastin.
    • To characterize the tropoelastin produced by these cells and its extracellular matrix deposition.
    • To establish a cell culture model for studying elastin synthesis regulation in the lung interstitium.

    Main Methods:

    • Isolation and culture of pulmonary interstitial fibroblasts from neonatal rat lungs.

    Related Experiment Videos

  • Biochemical, immunological, and ultrastructural analyses to assess elastin synthesis.
  • Amino acid analysis to characterize alkali-resistant elastin formation.

    • Main Results:

    • Fibroblasts synthesized a 75,000-dalton tropoelastin.
    • Soluble elastin was produced early, with insoluble elastin deposition beginning shortly after confluency.
    • Alkali-resistant elastin formation was chemically defined at 4 weeks post-confluency.
    • Tropoelastin's molecular weight matched that found in neonatal lung organ cultures.

    Conclusions:

    • Neonatal rat pulmonary fibroblast cultures effectively model elastin synthesis in the lung interstitium.
    • These cultures provide a valuable system for studying the regulation of elastin production and response.
    • The findings contribute to understanding the temporal dynamics of elastin deposition in lung development.