Identifying drug targets with thermal proteome profiling using IBT-16plex.

Area of Science:

• Chemical Biology
• Proteomics
• Drug Discovery

Background:

• Thermal proteome profiling (TPP) is a key technique for identifying drug targets.
• TMTpro-16plex facilitates TPP by enabling single-batch processing of multiple samples.
• A novel isobaric labeling reagent, IBT-16plex, was developed to enhance TPP performance.

Purpose of the Study:

• To evaluate the efficacy of the novel IBT-16plex reagent for drug target identification using TPP.
• To compare the performance of IBT-16plex against the established TMTpro-16plex reagent.

Main Methods:

• Application of IBT-16plex for thermal proteome profiling (TPP).
• Target identification for methotrexate and panobinostat.
• Analysis of protein thermal stability shifts to identify drug-target interactions.

Main Results:

• Successful identification of known drug targets for methotrexate and panobinostat.
• Detection of elevated melting temperatures for identified targets.
• Identification of known and potential novel drug off-targets.

Conclusions:

• IBT-16plex demonstrates superior or comparable performance to TMTpro-16plex in TPP.
• IBT-16plex is a viable and cost-effective alternative to TMTpro-16plex for TPP applications.
• The study validates IBT-16plex for robust drug target and off-target identification.