Related Experiment Video

Updated: Jul 8, 2025

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
08:12

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

5.5K

microRNAs Regulate Survivin in Colorectal Cancer Patients

Hadi Chavoshi1, Soghra Bornehdeli2, Milad Asadi3

  • 1Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Advanced Biomedical Research
|December 11, 2023
PubMed
Abstract

Insights

MicroRNA-203 (miR-203) is downregulated in colorectal cancer (CRC) and inversely correlates with survivin expression. This suggests miR-203 may play an oncogenic role in CRC progression, impacting lymph node metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Survivin is a key protein promoting tumor cell survival.
  • Dysregulation of microRNAs (miRNAs) is implicated in colorectal cancer (CRC) pathogenesis.
  • Understanding miRNA targets of survivin is crucial for CRC research.

Purpose of the Study:

  • To profile microRNAs (miRNAs) targeting survivin in Iranian colorectal cancer (CRC) patients.
  • To investigate the relationship between specific miRNAs and survivin expression in tumoral and marginal tissues.
  • To explore the potential role of miR-203 in CRC progression and metastasis.

Main Methods:

  • Quantitative Real-time PCR was used to determine transcript levels of survivin and specific miRNAs (miR-34a, miR-16, miR-150, miR-203a).
  • Analysis was performed on tumoral and marginal tissues from 50 Iranian CRC patients.
  • Statistical correlation and comparison analyses were conducted to assess expression levels and relationships.

Main Results:

  • Survivin mRNA expression was significantly elevated in tumoral tissues compared to marginal tissues (fold change = 3.21, P = 0.0029).
  • miR-16 and miR-203a showed significant downregulation in tumoral samples (fold change = 0.28, P = 0.003 and fold change = 0.36, P = 0.014, respectively).
  • A significant inverse correlation was observed between miR-203a and survivin expression (rho = -0.81; P < 0.001).
  • Higher survivin and miR-203 expression levels were noted in CRC patients with lymph node metastasis.

Conclusions:

  • miR-203 may function as an oncogene in colorectal cancer (CRC).
  • The observed downregulation of miR-203 and its inverse correlation with survivin suggest a regulatory role.
  • miR-203's association with lymph node metastasis indicates its potential involvement in CRC progression.
Keywords:
ApoptosisSurvivincolorectal cancermetastasismicroRNA

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.6K
An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.4K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.1K