Related Experiment Video
Updated: Jul 8, 2025
10:00
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
Published on: May 23, 2018
17.6K
The Principles and Applications of High-Throughput Sequencing Technologies
1School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, Korea.
Development & Reproduction
|December 11, 2023
Summary
High-throughput sequencing (HTS) advances biological research by enabling rapid data generation for genomics and disease studies. This review simplifies HTS technologies and their diverse applications for researchers new to the field.
Area of Science:
- Genomics and Molecular Biology
- Bioinformatics and Computational Biology
Background:
- High-throughput sequencing (HTS) has transformed biological research over the past two decades.
- It enables rapid generation of vast genetic data, crucial for genomics, epigenomics, transcriptomics, and metagenomics.
- Despite its widespread use, HTS technologies can present challenges for new researchers.
Purpose of the Study:
- To provide a comprehensive overview of current high-throughput sequencing technologies.
- To detail the applications of HTS in various biological research areas.
- To aid researchers, particularly newcomers, in understanding and utilizing HTS effectively.
Main Methods:
- Review of commonly used high-throughput sequencing platforms.
- Analysis of HTS applications across multiple biological domains.
- Synthesis of information for clarity and accessibility.
Main Results:
- Detailed explanation of HTS principles and methodologies.
- Exploration of HTS applications in genome sequencing, transcriptome analysis, DNA methylation studies, DNA-protein interactions, chromatin accessibility, 3D genome organization, and microbiome analysis.
- Identification of key advancements and challenges in HTS.
Conclusions:
- High-throughput sequencing is an indispensable tool in modern biological research.
- Understanding HTS technologies and their applications is vital for scientific advancement.
- This review serves as a foundational guide for researchers engaging with HTS data.
Related Concept Videos
03:21RNA-seq
10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K
03:00Next-generation Sequencing
89.0K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
89.0K
01:57Sanger Sequencing
754.5K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
754.5K
01:05Maxam-Gilbert Sequencing
11.2K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Challenges of the Maxam-Gilbert Method
The...
11.2K
02:02Genomics
36.4K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
36.4K
02:34DNA Microarrays
17.4K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
17.4K