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RNA polymerase II transcription with partially assembled TFIID complexes.
Vincent Hisler1,2,3,4, Paul Bardot1,2,3,4, Dylane Detilleux1,2,3,4
1Université de Strasbourg, IGBMC UMR 7104- UMR-S 1258, F-67400 Illkirch, France.
Biorxiv : the Preprint Server for Biology
|December 11, 2023
Summary
Partially assembled TFIID complexes, lacking TAF7 or TAF10, can initiate RNA polymerase II transcription. However, these TFIID variants do not fully replace holo-TFIID during development.
Area of Science:
- Molecular Biology
- Gene Regulation
- Developmental Biology
Background:
- RNA polymerase II (Pol II) transcription initiation is crucial for gene expression.
- The general transcription factor TFIID recognizes core promoter sequences.
- Metazoan holo-TFIID comprises TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs).
Conclusions:
- Partially assembled TFIID complexes are capable of sustaining basal Pol II transcription initiation.
- Complete holo-TFIID function is essential for long-term cellular viability and development.
- TAF7 and TAF10 are critical for the integrity and full functionality of TFIID beyond initial transcription steps.
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