Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Phosphatidylinositol diphosphate binding by ESCRT-III filaments.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Alternative organelle targeting of OPA1 mediates fatty acid release from lipid droplets.

bioRxiv : the preprint server for biology·2026

Same author

A multimodal adaptive optical microscope for in vivo imaging from molecules to organisms.

Nature methods·2026

Same author

Rational engineering of the P5 TRS-mimic site and REP78/68 start codon yields promoter variants that improve rAAV purity while maintaining high titers.

Molecular therapy. Advances·2026

Same author

Indoor pollution induced oxinflammatory responses in lung tissues.

Chemico-biological interactions·2026

Same author

Improved sensors for fructose-1,6-bisphosphate enable in vivo imaging of glycolysis.

bioRxiv : the preprint server for biology·2026

Same journal

Genetic Impacts on Variability of Body Fat Distribution Uncover Gene-Environment and Gene-Gene Interactions.

bioRxiv : the preprint server for biology·2026

Same journal

16S ribosomal RNA modification drives transcript-specific translation efficiency.

bioRxiv : the preprint server for biology·2026

Same journal

FlcE latches onto the FliL-stator complex to turbocharge flagellar motility in <i>Borrelia burgdorferi</i>.

bioRxiv : the preprint server for biology·2026

Same journal

Synaptic pruning, myelination and the emergence of psychiatric disorders in late adolescence.

bioRxiv : the preprint server for biology·2026

Same journal

Structural and functional insights into the Rcs phosphorelay.

bioRxiv : the preprint server for biology·2026

Same journal

The structural basis of RanGAP1 regulation and catalysis in nuclear transport.

bioRxiv : the preprint server for biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 8, 2025

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

Published on: October 13, 2019

A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction.

Xiao Li¹, Rico Gamuyao¹, Ming-Lun Wu¹

¹Department of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, United States.

Biorxiv : the Preprint Server for Biology

|December 11, 2023

Summary

This summary is machine-generated.

We developed FABCON, a new tool to visualize cellular contact sites between lipid droplets and organelles in real-time. This method reveals dynamic interactions crucial for cell energy and lipid regulation.

More Related Videos

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

A Fluorescence-based Assay of Phospholipid Scramblase Activity

A Fluorescence-based Assay of Phospholipid Scramblase Activity

Published on: September 20, 2016

Related Experiment Videos

Last Updated: Jul 8, 2025

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

Published on: October 13, 2019

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

A Fluorescence-based Assay of Phospholipid Scramblase Activity

A Fluorescence-based Assay of Phospholipid Scramblase Activity

Published on: September 20, 2016

Area of Science:

Cell Biology
Biophysics
Metabolic Regulation

Background:

Organelle contact sites are critical for cellular lipid and energy balance.
Visualizing these nanoscale interactions in living cells over time presents significant technical challenges.

Conclusions:

FABCON provides new insights into the dynamic regulation of lipid droplet-organelle contact sites.
The tool is generalizable for visualizing various organelle contact sites, including ER-mito.
Findings generate hypotheses for further mechanistic studies on metabolic regulation.