Picric Acid Incorporated Fluorescent Nitrogen Doped Carbon Dot for "Turn-On" Detection of Glutathione

Glutathione (GSH), a non-protein thiol in living cells whose abnormal level indicates the onset of diseases like Alzheimer's, HIV, diabetes, cancer, Parkinson's, Dementia, etc. Herein, we have synthesized a low-cost, selective, and sensitive detection platform by using citric acid and urea-derived fluorescent carbon dots (NCDs) via the microwave-assisted method, showing fluorescence at 444 nm. This fluorescence was quenched with picric acid (PA), and this probe, picric acid incorporated nitrogen doped carbon dot (NCDs@PA) was further used for the detection of GSH. The characterization of the probe was done by photoluminescence study, UV-Visible absorption studies, ATR-FTIR, SEM, and DLS analysis. GSH induced fluorescence recovery due to the competitive binding of GSH to PA. GSH was detected within a linear range of 0.31 mM- 2.43 mM with a Limit of Detection (LoD) and Limit of Quantification (LoQ) of 32.10 µM and 107.32 µM, respectively. The sensor exhibited good selectivity and sensitivity towards GSH among various co-existing ions and biomolecules. The paper-strip-based sensing of glutathione was conducted to check practical applicability of the probe, and a real sample analysis was also conducted from spiked human samples.