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G-POSS connected double network starch gels for protein release.

Seyma Nur Kirmic Cosgun1, Deniz Ceylan Tuncaboylu1, Mahinur Alemdar1

  • 1Bezmialem Vakıf University Health Sciences Institute, Department of Biotechnology, 34093 Istanbul, Turkey; Bezmialem Vakif University, Faculty of Pharmacy, 34093 Istanbul, Turkey.

International Journal of Biological Macromolecules
|December 11, 2023
PubMed
Summary

This study introduces novel starch-based nanocomposite hydrogels with enhanced mechanical strength. These advanced materials show promise for controlled protein release applications.

Keywords:
Double networkGlycidyl-POSSHydrogelNanocompositeProtein releaseStarch

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Area of Science:

  • Polymer Science
  • Materials Science
  • Biomaterials Engineering

Background:

  • Starch is a widely used natural polymer for hydrogel synthesis.
  • Developing mechanically robust hydrogels is crucial for advanced applications.
  • Combining brittle and ductile networks with inorganic particles can enhance hydrogel properties.

Purpose of the Study:

  • To design and synthesize mechanically enhanced nanocomposite double network (DN) starch gels.
  • To incorporate inorganic particles and create dual networks for improved material properties.
  • To evaluate the potential of these starch nanocomposite gels for protein release.

Main Methods:

  • Synthesized nanocomposite starch gels (s-NC) by cross-linking starch with 8-armed glycidyl-polyhedral oligomeric silsesquioxane (g-POSS).
  • Incorporated N,N-dimethylacrylamide (DMA) or 1-vinyl-2-pyrrolidinone (VP) to form d-NC-DMA and d-NC-VP gels.
  • Characterized gel structure using Fourier Transform Infrared Spectroscopy and Energy Dispersive X-Ray Spectroscopy.
  • Analyzed morphology via SEM and mechanical properties through rheological tests, compression, and tensile analyses.
  • Demonstrated in vitro protein release using BSA and amylase sensitivity.

Main Results:

  • Successfully synthesized starch nanocomposite gels (s-NC) with homogeneously distributed g-POSS.
  • Developed more stable d-NC-DMA and d-NC-VP gels with enhanced mechanical strength compared to s-NC.
  • d-NC-DMA exhibited smaller pores and thicker pore walls, correlating with superior mechanical performance.
  • Confirmed the potential for controlled protein (BSA) release from the starch gels.

Conclusions:

  • Developed novel mechanically superior starch nanocomposite double network hydrogels.
  • g-POSS cross-linking and dual network formation significantly enhance mechanical properties.
  • These starch-based nanocomposite gels are promising for applications in controlled protein delivery systems.