Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Negative Regulator Molecules01:23

Negative Regulator Molecules

35.4K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.4K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

4.7K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
4.7K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K
Replication in Eukaryotes01:29

Replication in Eukaryotes

13.8K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
13.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Autosomal allelic inactivation at loci with variable replication timing and dosage sensitivity.

eLife·2026
Same author

Cortical development dynamics across autism spectrum disorder mouse models.

Nature·2026
Same author

A human induced pluripotent stem cell model for the holistic study of epithelial-to-mesenchymal transitions.

Nature methods·2026
Same author

Transcription elongation can be sufficient, but is not necessary, to advance replication timing.

EMBO reports·2026
Same author

HairTime: A noninvasive assay for estimating circadian phase from a single hair sample.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Recombination junctions from antibody isotype switching classify immune and DNA repair dysfunction.

Nature communications·2025

Related Experiment Video

Updated: Jul 8, 2025

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

14.0K

RIF1 regulates early replication timing in murine B cells.

Daniel Malzl1,2, Mihaela Peycheva1,2, Ali Rahjouei3

  • 1Research Institute of Molecular Pathology (IMP), Vienna Biocenter, 1030, Vienna, Austria.

Nature Communications
|December 11, 2023
PubMed
Summary

RIF1 protein promotes early DNA replication in B lymphocytes by binding active chromatin. It works with MCM proteins to ensure timely replication of essential genes, revealing new regulatory layers in B cell genomes.

More Related Videos

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.5K
Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

39.9K

Related Experiment Videos

Last Updated: Jul 8, 2025

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

14.0K
Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.5K
Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

39.9K

Area of Science:

  • Genomics
  • Molecular Biology
  • Cell Biology

Background:

  • DNA replication timing (RT) is vital for genome stability.
  • RIF1 protein is known to suppress late replication origins in heterochromatin.

Purpose of the Study:

  • To investigate RIF1's role in DNA replication timing in antigen-activated murine B lymphocytes.
  • To explore the interplay between RIF1 and MCM proteins in regulating B cell RT.

Main Methods:

  • Analysis of RIF1 binding patterns in B lymphocytes.
  • Assessment of RIF1's impact on replication origin activity, gene expression, and genome organization.
  • Investigating the functional relationship between RIF1 and minichromosome maintenance (MCM) proteins.

Main Results:

  • RIF1 predominantly binds early-replicating active chromatin in B cells, promoting early replication.
  • RIF1 has a limited role in regulating origin activity, gene expression, and genome organization in this context.
  • RIF1 and MCM proteins act complementarily to establish early RT signatures and ensure timely replication of highly transcribed genes.

Conclusions:

  • RIF1 plays a significant role in promoting early replication in B cells, distinct from its heterochromatin-associated function.
  • The coordinated action of RIF1 and MCM proteins introduces novel regulatory mechanisms to the B cell replication timing program.
  • These findings deepen our understanding of genome regulation during B cell activation and differentiation.