Related Experiment Video
Updated: Jul 8, 2025

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells
Published on: March 7, 2025
Re-"Formate" T-cell Antitumor Responses
Mei-Chun Lin1,2,3, Sofie Hedlund Moller1,3, Ping-Chih Ho1,3
1Department of Oncology, University of Lausanne, Lausanne, Switzerland.
Summary:
Rowe and colleagues discover that one-carbon (1C) metabolism rewiring occurs upon T-cell activation to support proliferation and cytolytic activity in CD8+ T cells and that supplementation of 1C donor formate rescues the dysfunctional T cells and their responsiveness to anti-PD-1 in selective tumor-infiltrated T-cell subsets. This finding represents an attractive strategy to overcome a metabolic vulnerability in the tumor microenvironment and improve the efficacy of immune checkpoint blockade. See related article by Rowe et al., p. 2566 (8).
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