Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

X-ray Crystallography

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...

$X-ray Diffraction of Biological Samples$ 01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...

Scanning Electron Microscopy

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...

X-ray Imaging

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multifunctional bending magnet beamline with a capillary optic for X-ray fluorescence studies of metals in tissue sections.

Journal of synchrotron radiation·2026

Same author

Multifunctional bending magnet beamline with a capillary optic for X-ray fluorescence studies of metals in tissue sections.

bioRxiv : the preprint server for biology·2025

Same author

3D imaging of magnetic domains in Nd<sub>2</sub>Fe<sub>14</sub>B using scanning hard X-ray nanotomography.

Journal of synchrotron radiation·2024

Same author

Unsupervised classification for region of interest in X-ray ptychography.

Scientific reports·2023

Same author

Correlating chemistry and mass transport in sustainable iron production.

Proceedings of the National Academy of Sciences of the United States of America·2023

Same author

Counting on the future: fast charge-integrating detectors for X-ray nanoimaging.

Journal of synchrotron radiation·2023

Search research articles

Related Experiment Video

Updated: Jun 17, 2026

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

Efficient boundary-guided scanning for high-resolution X-ray ptychography.

Dergan Lin¹, Yi Jiang², Junjing Deng²

¹Mathematics and Computer Science Division, Argonne National Laboratory, Lemont, IL 60439, USA.

Journal of Synchrotron Radiation

|December 12, 2023

Summary

This study introduces an algorithm for X-ray ptychography that intelligently guides data acquisition. It reduces redundant measurements by focusing on the sample, improving efficiency and reconstruction time.

Keywords:

X-ray ptychography automated data acquisition

More Related Videos

$High Pressure Single Crystal Diffraction at PX^2$

High Pressure Single Crystal Diffraction at PX^2

Published on: January 16, 2017

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

Published on: June 12, 2020

Related Experiment Videos

Last Updated: Jun 17, 2026

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

$High Pressure Single Crystal Diffraction at PX^2$

High Pressure Single Crystal Diffraction at PX^2

Published on: January 16, 2017

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

Published on: June 12, 2020

Area of Science:

Materials Science
Physics
Imaging Science

Background:

X-ray ptychography is crucial for high-resolution sample reconstruction.
Conventional methods involve extensive scanning, including redundant background measurements.
Inefficient data acquisition prolongs experiment and reconstruction times.

Purpose of the Study:

To develop an automated algorithm for efficient X-ray ptychography data acquisition.
To reduce the number of measurements while maintaining high-resolution reconstructions.
To optimize the scanning process for time and resource savings.

Main Methods:

An innovative algorithm guides scan points to actively locate the sample.
The algorithm performs intelligent perimeter scanning, focusing on the region of interest.
Measurements are strategically acquired only within the sample's boundary.

Main Results:

Demonstrated reduction in the number of required measurements compared to raster scanning.
Significant time savings achieved during the data reconstruction process.
Validation of the algorithm's efficacy through practical implementation in real experiments.

Conclusions:

The proposed algorithm enhances the efficiency and accuracy of X-ray ptychography.
Automated guidance minimizes redundant data collection, optimizing experimental workflows.
This approach has potential for advancing sample analysis and imaging across scientific disciplines.