Related Experiment Video

Updated: Jul 8, 2025

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
04:46

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

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Trim21 Regulates the Postnatal Development and Thermogenesis of Brown Adipose Tissue

Qinxiao Hu1, Yidi Xu1, Teng Xiao1

  • 1Department of Bone and Joint Surgery, the First Affiliated Hospital of Jinan University, Key Laboratory of Regenerative Medicine of Ministry of Education of Jinan University, Guangzhou, Guangdong, 510630, China.

Advanced Biology
|December 12, 2023
PubMed

Insights

Tripartite motif containing 21 (Trim21) is vital for brown adipose tissue development and function. Trim21 deficiency impairs thermogenesis, reduces energy expenditure, and exacerbates diet-induced obesity.

Area of Science:

  • Metabolism and Endocrinology
  • Adipose Tissue Biology
  • Obesity Research

Background:

  • Brown adipose tissue (BAT) is crucial for thermoregulation and energy expenditure.
  • Postnatal development of BAT is essential for protection against cold and obesity.
  • The molecular mechanisms regulating BAT maturation and function require further elucidation.

Purpose of the Study:

  • To investigate the role of Tripartite motif containing 21 (Trim21) in brown adipose tissue (BAT) development and thermogenesis.
  • To determine the impact of Trim21 deficiency on energy expenditure and susceptibility to diet-induced obesity.

Main Methods:

  • Analysis of Trim21 mRNA expression in interscapular BAT during postnatal development.
  • Assessment of BAT maturation, thermogenic gene expression, and energy expenditure in Trim21-deficient mice.
  • Evaluation of white adipose tissue browning and obesity development in response to cold exposure, a β-adrenergic agonist, and high-fat diet.

Main Results:

  • Trim21 mRNA levels in interscapular BAT increase postnatally, peaking at postnatal day 14.
  • Trim21 depletion impairs BAT maturation, reduces thermogenic gene expression, and lowers energy expenditure.
  • Loss of Trim21 suppresses white adipose tissue browning and increases susceptibility to high-fat diet-induced obesity.

Conclusions:

  • Trim21 plays a critical role in regulating the postnatal development of brown adipose tissue.
  • Trim21 is essential for maintaining thermogenesis and energy expenditure.
  • Trim21 deficiency contributes to obesity, highlighting its importance in metabolic homeostasis.

Keywords:
Trim21adipose tissuebrowningpostnatal developmentthermogenesis

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