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Updated: Jul 8, 2025

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Published on: December 11, 2021
Fluorescence of p-hydroxyazobenzocrowns - Tautomeric equilibrium effect
Paulina Szulc1, Elżbieta Luboch1, Andrzej Okuniewski2
1Department of Chemistry and Technology of Functional Materials, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza Street 11/12, 80-233 Gdańsk, Poland.
Abstract:
The spectroscopic properties of a series of para-hydroxyazobenzocrowns, including three novel compounds, were investigated using UV-Vis absorption and emission spectroscopy. This study presents, for the first time, determined quantum yield (QY) values for macrocycles of this category, ranging between 0.122 and 0.195. The highest values were obtained for crowns bearing two phenyl substituents in benzene rings. The impact of aromatic ring substituents and macroring size on the spectral characterization (1H NMR and FTIR) of p-hydroxyazobenzocrowns was examined in consideration of the azophenol ⇄ quinone-hydrazone tautomeric equilibrium. Dipole moments of p-hydroxyazobenzocrowns in the ground and excited states have been determined. The alignment between experimental findings and theoretical studies was established.
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