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Related Experiment Video

Updated: Jul 8, 2025

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

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Bond-selective full-field optical coherence tomography.

Haonan Zong, Celalettin Yurdakul, Jian Zhao

    Optics Express
    |December 13, 2023
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces bond-selective full-field Optical Coherence Tomography (BS-FF-OCT), a novel technique for label-free, 3D chemical imaging. BS-FF-OCT successfully visualizes diverse samples, from polymer beads to complex biological tissues.

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    Area of Science:

    • Biomedical Optics
    • Spectroscopy
    • 3D Imaging

    Background:

    • Optical Coherence Tomography (OCT) provides label-free, non-invasive 3D imaging for research and clinical use.
    • Conventional OCT lacks chemical information, limiting its diagnostic capabilities.
    • Developing new OCT modalities for chemical analysis is crucial for advanced biological and medical applications.

    Purpose of the Study:

    • To develop and demonstrate a novel bond-selective full-field OCT (BS-FF-OCT) technique.
    • To achieve label-free, 3D chemical contrast in highly scattering samples.
    • To expand the application scope of OCT beyond structural imaging.

    Main Methods:

    • Utilized a pulsed mid-infrared laser to induce photothermal modulation of the OCT signal.
    • Implemented a bond-selective full-field OCT (BS-FF-OCT) system.
    • Applied the technique to various samples including polymer beads, surgical masks, cell spheroids, C. elegans, and mouse brain tissue.

    Main Results:

    • Successfully performed label-free, 3D bond-selective imaging of 1 µm PMMA beads in agarose gel.
    • Achieved 3D hyperspectral imaging of polypropylene fibers from a surgical mask.
    • Demonstrated BS-FF-OCT on biological samples like cancer cell spheroids and C. elegans.
    • Visualized highly scattering myelinated axons in mouse brain tissue using an optimized pulse timing configuration.

    Conclusions:

    • BS-FF-OCT enables label-free, bond-selective 3D imaging of scattering samples.
    • The technique offers valuable chemical information complementary to structural OCT data.
    • BS-FF-OCT shows significant potential for diverse applications in biological research and clinical diagnostics.