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Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
Published on: November 11, 2016
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Circadian protein expression patterns in healthy young adults
Adrien Specht1, German Kolosov1, Katie L J Cederberg1
1Department of Psychiatry and Behavioral Sciences, Stanford University, Palo Alto, California, USA.
Sleep Health
|December 13, 2023
Summary
Hundreds of blood plasma proteins show daily rhythms in humans, revealing new insights into circadian biology and personalized medicine. This study identified over a thousand rhythmic proteins, many with consistent daily patterns.
Area of Science:
- Chronobiology
- Proteomics
- Human Physiology
Background:
- Circadian rhythms regulate numerous physiological processes.
- Understanding daily fluctuations in blood plasma proteins is crucial for human health.
- Previous studies have hinted at circadian variations in the proteome, but comprehensive analysis is lacking.
Purpose of the Study:
- To investigate the 24-hour fluctuations of the blood plasma proteome.
- To identify specific proteins exhibiting endogenous circadian rhythmicity.
- To explore the implications for circadian medicine.
Main Methods:
- Hourly plasma sampling from 17 healthy adults under controlled conditions and a typical sleep-wake schedule.
- Quantitative proteomic analysis of 6916 proteins using the SomaScan platform.
- Statistical analysis using a cosinor model with mixed effects to detect rhythmicity.
Main Results:
- 1063 proteins (15%) demonstrated significant daily rhythmicity.
- 431 proteins (6.2%) showed consistent endogenous circadian rhythms under both conditions.
- A significant number of rhythmic proteins peaked in the late afternoon/evening or early morning.
Conclusions:
- Hundreds of plasma proteins exhibit endogenous circadian rhythmicity in humans.
- This finding opens avenues for discovering novel circadian-regulated pathways.
- It supports the integration of circadian rhythm knowledge into personalized and predictive medicine.
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