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Related Concept Videos

G Protein-coupled Receptors01:15

G Protein-coupled Receptors

12.1K
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
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Transducer Mechanism: G Protein–Coupled Receptors01:30

Transducer Mechanism: G Protein–Coupled Receptors

2.0K
G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical,...
2.0K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.3K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K

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Related Experiment Video

Updated: Jul 8, 2025

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay
09:12

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

Published on: September 10, 2016

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Microscopy and spectroscopy approaches to study GPCR structure and function.

Tomáš Fessl1, Maria Majellaro2, Alexey Bondar1,3

  • 1Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.

British Journal of Pharmacology
|December 13, 2023
PubMed
Summary
This summary is machine-generated.

Advanced optical microscopy and spectroscopy enable detailed observation of G-protein coupled receptor (GPCR) signaling pathways in living systems. These techniques offer high resolution for studying cellular signaling with minimal disruption.

Keywords:
G proteinG protein‐coupled receptorbiosensorscell signallingmicroscopyspectroscopysuper‐resolution

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In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
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In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

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Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization
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Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization

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Last Updated: Jul 8, 2025

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay
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G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

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In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
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Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization
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Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization

Published on: March 16, 2020

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Area of Science:

  • Cellular Biology
  • Biophysics
  • Biochemistry

Background:

  • G-protein coupled receptors (GPCRs) mediate signal transduction for diverse stimuli like light, odorants, neurotransmitters, and hormones.
  • Understanding GPCR structural and functional dynamics necessitates high-resolution, real-time observation of signaling cascades.
  • Endogenous systems and living cells require non-perturbing investigation methods.

Purpose of the Study:

  • To review recent advancements in optical microscopy and spectroscopy for GPCR signaling research.
  • To highlight techniques offering high spatial and temporal resolution for studying cellular signaling.
  • To guide researchers in selecting optimal imaging and spectroscopy methods for GPCR studies.

Main Methods:

  • Review of advanced optical microscopy techniques (e.g., super-resolution microscopy).
  • Exploration of spectroscopy methods for probing molecular dynamics.
  • Discussion of single-molecule detection strategies.
  • Analysis of labeling techniques for endogenous systems.
  • Consideration of methods applicable to large living organisms.

Main Results:

  • Recent developments provide unprecedented insights into GPCR signaling mechanisms.
  • Advanced techniques achieve high spatial and temporal resolution, enabling detailed observation.
  • Single-molecule methods and improved labeling strategies facilitate studies in endogenous systems.
  • Applicability to large living objects expands the scope of GPCR research.

Conclusions:

  • Optical microscopy and spectroscopy are essential tools for dissecting GPCR signaling.
  • The reviewed technologies empower researchers to investigate complex cellular signaling pathways.
  • This review aids in the strategic selection of advanced imaging and spectroscopy techniques for GPCR research.