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The peroxisome protein translocation machinery is developmentally regulated in the fungus Podospora anserina

Beatriz Aguirre-López1, Fernando Suaste-Olmos1, Leonardo Peraza-Reyes1

  • 1Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México , Coyoacán, Mexico.

Microbiology Spectrum
|December 13, 2023
PubMed
Abstract

Insights

Meiosis in eukaryotes involves peroxisome regulation. The protein PEX13 is selectively removed during sexual reproduction, impacting peroxisome function and cell rejuvenation.

Area of Science:

  • Cell biology
  • Mycology
  • Genetics

Background:

  • Sexual reproduction in eukaryotes generates genetic diversity through meiosis.
  • Meiosis requires precise regulation of cellular processes, including organelle function.
  • Peroxisomes are crucial for meiosis induction and development in the model fungus *Podospora anserina*.

Purpose of the Study:

  • To investigate the developmental regulation of peroxisomes during meiosis.
  • To identify key proteins involved in peroxisome function during sexual reproduction.
  • To understand the role of PEX13 in meiotic development.

Main Methods:

  • Analysis of PEX13 protein levels during meiotic development.
  • Investigation of ubiquitination-dependent protein degradation pathways.
  • Studying peroxisome dynamics and function in *Podospora anserina*.

Main Results:

  • PEX13, essential for peroxisome protein import, is developmentally regulated during meiosis.
  • PEX13 abundance is modulated through selective ubiquitination-dependent removal.
  • This regulation impacts the peroxisome protein translocation system throughout meiotic development.

Conclusions:

  • Meiotic development involves intricate control over the peroxisome protein import machinery.
  • Selective degradation of PEX13 is a key mechanism for regulating peroxisome function during sexual reproduction.
  • Understanding these mechanisms contributes to knowledge of eukaryotic sexual reproduction and cell rejuvenation.
Keywords:
Podosporafilamentous fungimeiosisorganelle protein importperoxisomessexual development

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