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Spatial tumor biopsy with fluorescence PCR microneedle array.

Xiaoxuan Zhang1, Guopu Chen1, Yu Wang1

  • 1Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

Innovation (Cambridge (Mass.))
|December 13, 2023
PubMed
Summary
This summary is machine-generated.

A novel microneedle array (MNA) platform enables efficient spatial tumor biopsy for enhanced cancer diagnosis. This fluorescence polymerase chain reaction (F-PCR) MNA platform simplifies tumor biomarker detection in various samples.

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Area of Science:

  • Biotechnology
  • Materials Science
  • Oncology

Background:

  • Biopsy is crucial for accurate tumor diagnosis, providing detailed insights into tumorigenesis and progression.
  • Current biopsy methods can be invasive and may not always capture spatial heterogeneity effectively.

Purpose of the Study:

  • To develop an efficient spatial tumor biopsy platform using a microneedle array (MNA) for fluorescence polymerase chain reaction (F-PCR).
  • To enable rapid and stereoscopic collection of tumor nucleic acid biomarkers for cancer marker discovery.

Main Methods:

  • Fabrication of MNA using bottom-up self-assembly and top-down photolithography.
  • Utilizing a hydrophobic silica nanoparticle-assembled substrate and graphene aerogel-hydrogel hybrid microneedle peaks.
  • Employing discrete wettability for efficient fluid management and direct F-PCR reactions on microneedle peaks.

Main Results:

  • The F-PCR-MNA platform demonstrated efficient spatial tumor biopsy and stereoscopic collection of nucleic acid biomarkers.
  • Successful detection of DNA biomarkers for lung carcinoma in standard solutions, mouse tissues, and clinical specimens.
  • The platform's discrete wettability facilitated easy removal of tissue and PCR liquids, with each microneedle acting as an independent reaction site.

Conclusions:

  • The developed F-PCR-MNA platform offers a practical and innovative approach to tumor biopsy.
  • This technology holds significant potential for advancing tumor diagnosis and biomarker discovery.
  • The MNA system provides an efficient method for detecting cancer DNA biomarkers with potential clinical applications.