Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

51.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
51.6K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
Homologous Recombination02:31

Homologous Recombination

50.6K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mapping the evolution of sepsis and intestinal epithelial barrier research: a bibliometric analysis (1977-2024).

Journal of thoracic disease·2026
Same author

MKRN1 suppresses cuproptosis in colorectal cancer through ubiquitin-dependent regulation of SLC31A1.

Biological procedures online·2026
Same author

Impact of Subretinal Drusenoid Deposits on Ellipsoid Zone-Related Thickness Metrics.

Investigative ophthalmology & visual science·2026
Same author

4-Octyl itaconate attenuates renal calculi formation by inhibiting ferroptosis and oxidative stress via the Nrf2-HO-1/SLC7A11 axis.

European journal of pharmacology·2026
Same author

From targeted delivery to inflammation suppression: engineering plant exosomes for heart failure therapy post-infarction.

Materials today. Bio·2026
Same author

Therapeutic hypothermia protects the ischemic penumbra via Xkr8.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics·2026

Related Experiment Video

Updated: Jul 8, 2025

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
11:28

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

Published on: May 23, 2016

17.7K

CRISPR/Cas9 systems: Delivery technologies and biomedical applications.

Yimin Du1, Yanfei Liu2, Jiaxin Hu1

  • 1Department of Pharmaceutics, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China.

Asian Journal of Pharmaceutical Sciences
|December 13, 2023
PubMed
Summary

Efficient delivery of CRISPR/Cas9 gene editing is crucial for disease management and research. This review explores various physical, viral, and non-viral delivery strategies and their biomedical applications.

Keywords:
Biomedical applicationsCRISPR/Cas9Non-viral vectorPhysical deliveryViral vector

More Related Videos

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.2K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.0K

Related Experiment Videos

Last Updated: Jul 8, 2025

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
11:28

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

Published on: May 23, 2016

17.7K
A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.2K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.0K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Gene Editing

Background:

  • The CRISPR/Cas9 system offers revolutionary potential for disease management and creating animal models.
  • Efficient and secure delivery of CRISPR/Cas9 to target sites is essential for its therapeutic efficacy.
  • Developing advanced delivery methods is a critical research focus.

Purpose of the Study:

  • To provide a comprehensive overview of CRISPR/Cas9 delivery strategies.
  • To analyze various delivery methods, including physical, viral, and non-viral vectors.
  • To discuss the biomedical applications and future prospects of CRISPR/Cas9 delivery.

Main Methods:

  • Review of existing literature on CRISPR/Cas9 delivery systems.
  • In-depth analysis of physical, viral vector, and non-viral vector (plasmid, mRNA, protein-based) approaches.
  • Illustration of biomedical applications of the CRISPR/Cas9 system.

Main Results:

  • Detailed examination of diverse delivery strategies for CRISPR/Cas9.
  • Discussion of key factors influencing delivery efficiency and current challenges.
  • Highlighting the potential of various delivery methods in biomedical research.

Conclusions:

  • Effective delivery is paramount for harnessing the full potential of CRISPR/Cas9 gene editing.
  • Continued innovation in delivery strategies is needed to overcome current challenges.
  • Advancements in CRISPR/Cas9 delivery promise significant breakthroughs in biomedical research and therapeutics.