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Hematopoietic effect of echinochrome on phenylhydrazine-induced hemolytic anemia in rats
Mona S E F El-Shehry1, Rafa A Amrymi2, Tarek Atia3
1Biotechnology Department, Faculty of Biotechnology-October University for Modern Sciences and Arts (MSA), Cairo, Egypt.
Background:
Hemolytic anemia (HA) is a serious health condition resulting from reduced erythrocytes' average life span. Echinochrome (Ech) is a dark-red pigment found in shells and spines of sea urchins.
Aim:
Studying the potential therapeutic effect of Ech on phenylhydrazine (PHZ)-induced HA in rats.
Methods:
Eighteen rats were divided into three groups (n = 6): the control group, the phenylhydrazine-induced HA group and the Ech group, injected intraperitoneally with PHZ and supplemented with oral Ech daily for 6 days.
Results:
Ech resulted in a considerable increase in RBCs, WBCs, and platelets counts, hemoglobin, reduced glutathione, catalase, and glutathione-S-transferase levels, and a significant decrease in aspartate & alanine aminotransferases, alkaline phosphatase, gamma-glutamyl transferase, bilirubin, creatinine, urea, urate, malondialdehyde & nitric oxide levels in anemic rats. Histopathological examination of liver and kidney tissue samples showed marked improvement.
Conclusion:
Ech ameliorated phenylhydrazine-induced HA with a hepatorenal protective effect owing to its anti-inflammatory and antioxidant properties.
