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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.3K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.3K

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Updated: Jul 8, 2025

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
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Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

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Best practice: setting up and operating a mid-sized cryo-EM facility.

Xing Meng1,2, Ishara Ratnayake1,2, Martha L Escobar Galvis3

  • 1David Van Andel Advanced Cryo-Electron Microscopy Suite, Van Andel Institute, Grand Rapids, MI, United States.

Frontiers in Molecular Biosciences
|December 13, 2023
PubMed
Summary

Establishing a mid-sized cryo-electron microscopy (cryo-EM) facility requires careful planning, equipment selection, and IT solutions. This guide offers insights into optimizing operations, managing maintenance, and understanding the costs for high-performance single-particle cryo-EM.

Keywords:
IT-toolscore facilitycryo-EMinfrastructuremaintenanceoperationsetting up

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Area of Science:

  • Structural biology
  • Biophysics
  • Biochemistry

Background:

  • Cryo-electron microscopy (cryo-EM) has revolutionized structural biology since 2013, enabling detailed analysis of large, flexible molecular complexes.
  • The demand for cryo-EM facilities has surged globally, yet access to advanced instrumentation and expertise remains a significant barrier for many researchers.
  • Government agencies and institutions like the Van Andel Institute (VAI) have invested in cryo-EM centers to provide broader access and training.

Purpose of the Study:

  • To provide a comprehensive blueprint for establishing a new mid-sized cryo-electron microscopy facility.
  • To share practical strategies for optimizing routine operations for high performance and efficiency in single-particle cryo-EM.
  • To detail essential information on planning, equipment, IT solutions, common operational issues, and long-term maintenance costs.

Main Methods:

  • The study outlines key considerations for initial planning and equipment selection for a cryo-EM facility.
  • It discusses the development and implementation of IT solutions to enhance data collection and analysis workflows.
  • Operational challenges and maintenance requirements were tracked over a six-year period to inform cost estimations.

Main Results:

  • The paper details critical factors for establishing and operating a successful mid-sized cryo-EM facility.
  • It presents data on common issues and maintenance needs over six years, aiding in long-term cost projection.
  • Strategies for optimizing performance and efficiency in single-particle cryo-EM are discussed.

Conclusions:

  • Establishing a dedicated cryo-EM facility requires strategic planning, appropriate technology, and robust operational management.
  • This blueprint offers valuable insights for institutions considering the development of their own cryo-EM core facilities.
  • Understanding operational challenges and long-term costs is crucial for the sustainable success of cryo-EM services.