Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Spectrophotometry: Introduction

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Enzymatic Laccase Nanoreactors Induce Apoptosis in MOLT-4-ALL Cells and Activate Prodrugs in a Synergetic Effect.

Blood and lymphatic cancer : targets and therapy·2026

Same author

Effect of Zinc Oxide Nanoparticles From Infant Formula Milk Powder on Colon Cells In Vitro.

Journal of applied toxicology : JAT·2026

Same author

Catalytically active inclusion bodies of Bacillus clausii laccase protein recombinantly produced in E. coli for dye decolorization.

Microbial cell factories·2026

Same author

Monomeric Glycine oxidase from Azotobacter vinelandii for Glycine biosensing.

World journal of microbiology & biotechnology·2025

Same author

Degradation of Extra-Heavy Crude Oil by Fungi Isolated from Hydrothermal Vents Fields in the Gulf of California.

Mycobiology·2025

Same author

Nanoparticles in Antibacterial Therapy: A Systematic Review of Enhanced Efficacy against Intracellular Bacteria.

ACS omega·2025

Search research articles

Related Experiment Video

Updated: Jul 8, 2025

Author Spotlight: Eco-friendly Photoluminescent Textile Authentication with Curcumin

Author Spotlight: Eco-friendly Photoluminescent Textile Authentication with Curcumin

Published on: December 22, 2023

An accurate spectrophotometric method for chitosan quantification.

Oscar González-Davis¹, Itandehui Betanzo¹, Rafael Vazquez-Duhalt¹

¹Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Baja California, 22860, Mexico.

Biology Methods & Protocols

|December 13, 2023

Summary

This summary is machine-generated.

A new spectrophotometric method accurately determines chitosan concentration. This method uses chitosan deamination followed by anthrone reagent carbohydrate determination for reliable quantification.

Keywords:

anthrone carbohydrate chitosan determination deamination

More Related Videos

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Published on: October 26, 2016

Measurement of Chitinase Activity in Biological Samples

Measurement of Chitinase Activity in Biological Samples

Published on: August 22, 2019

Related Experiment Videos

Last Updated: Jul 8, 2025

Author Spotlight: Eco-friendly Photoluminescent Textile Authentication with Curcumin

Author Spotlight: Eco-friendly Photoluminescent Textile Authentication with Curcumin

Published on: December 22, 2023

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Published on: October 26, 2016

Measurement of Chitinase Activity in Biological Samples

Measurement of Chitinase Activity in Biological Samples

Published on: August 22, 2019

Area of Science:

Biochemistry
Analytical Chemistry

Background:

Chitosan, derived from chitin, is a highly abundant natural biopolymer.
Chitosan possesses diverse applications across biomedical, environmental, and industrial sectors.
Accurate quantification is crucial for optimizing chitosan's utility.

Purpose of the Study:

To develop and validate a novel spectrophotometric method for precise chitosan concentration determination.
To establish a reliable analytical technique for quantifying chitosan in various matrices.

Main Methods:

The method involves the deamination of chitosan using nitrite under acidic conditions.
Subsequent carbohydrate determination is performed using the anthrone reagent.
Spectrophotometry is employed for accurate measurement of the resulting colored complex.

Main Results:

The developed spectrophotometric method provides accurate and reliable quantification of chitosan concentration.
The procedure is sensitive and reproducible, suitable for diverse applications.
Validation data confirms the method's efficacy in determining chitosan levels.

Conclusions:

A new, accurate spectrophotometric method for chitosan quantification has been established.
This method offers a valuable tool for researchers and industries utilizing chitosan.
The technique enhances the ability to precisely measure chitosan concentration, supporting its widespread application.